Abstract
Surface plasmon resonance (SPR)
is an affinity based, label-free biophysical technique for the analysis of
biomolecular interactions, including the determination of the kinetic
parameters. To enable a better understanding of the interactions taking place,
better predictions and analysis, as well as reduced computing resources, it is
crucial to obtain accurate models and rate constants. Several attempts have
been reported in order to enhance the resolution of SPR systems. However, there is no clear and
straightforward procedure to choose and analyze the appropriate biphasic SPR
data.FIU inventors have developed new
methods of analyzing the biphasic SPR data using the three commonly employed
biphasic models. The methods are based on a general analytical solution of the
biphasic rate equations, which is much more transparent and straightforward
than the highly non-linear numerical integration approach. This method can be
used to determine the underlying biphasic interaction mechanism from the
analysis of the SPR data, and extract the rate constants with high confidence
levels.Benefit
Improves SPR analysisObtains more accurate models and rate constantsReduces computing resourcesProvides better predictions of biomolecular interactionsCan be adapted to existing data fitting software packageMarket Application
Drug discoveryFood and beverage quality and safetyLife scienceElectrochemical analysisEnvironmental scienceChemical sensor development
Biosciences
