Dramatically Increases Yields and Infectivity of Adeno-Associated Viral Vectors Using Media With Potassium and Sodium Chloride
This production of adeno-associated viral vectors using sodium chloride and potassium chloride mediate increases yields of highly infectious viral vectors up to 10-fold as compared to other production methods. The global viral vector manufacturing market is projected to reach $815.8 million by 2023. In particular, AAV vectors have clinical and research applications, including use in FDA-approved gene therapy drugs, because they allow for long-term gene expression. However, widespread clinical use of AAV vectors has been limited due to production bottlenecks and failure to meet the manufacturing demands required for clinical trials.
Researchers at the University of Florida have developed a production system that generates high yields of AAV viral vectors. This system has demonstrated increased yields up to 10-fold as compared to other production methods, offering a potential solution to low-performing production systems that have limited the clinical use of AAV vectors for gene therapy.
Application
High-yield AAV vector production system that provides a scalable solution for high-titer and high-quality vectors using the herpes simplex virus platform
Advantages
- Increases yields of AAV vectors, addressing production bottleneck and allowing for widespread availability in clinical gene therapy applications
- Improves infectivity of AAV vectors, increasing efficacy of viral vectors for gene therapy
- Provides the structure for a scalable system, enabling large-scale manufacturing
Technology
This high-yield AAV vector production system is scalable and can increase yields up to 10-fold as compared to other production systems. This system increases yields and infectivity of AAV vectors in mammalian cells by manipulating the concentrations of potassium and sodium chloride in the production medium. The salt concentrations can be altered before, during, or after mammalian cells make contact with a viral vector such as the herpes simplex virus.
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