Research Terms
2013
1. Cornett E.M., O’steen M.R, Kolpashchikov D.M. (2013) Operating cooperatively (OC) sensor for highly specific recognition of nucleic acids. PLOS one, PLoS ONE 8(2): e55919.
2. Cornett E.M., Gerasimova Y.V., Kolpashchikov D.M.* (2013) A two-component reagent: an approach to specific covalent inactivation of biopolymers. Bioorganic & Medicinal Chemistry, 21, 1988–1991.
3. Gerasimova Y.V.,* Kolpashchikov D.M.* (2013) Detection of bacterial 16S rRNA using a molecular beacon-based X sensor. Biosensors & Bioelectronics, 41, 386–390.
2012
1. Cornett E.M., Campbell E.A., Gulenay G., Peterson E, Bhaskar N, Kolpashchikov D.M. (2012) Molecular logic gates for DNA analysis: detection of rifampin resistance in M.tuberculosis DNA Angew Chem Int Ed Engl. (Accepted)
3. Kolpashchikov D.M. (2012) An elegant biosensor molecular beacon probe: challenges and recent solutions, Scientifica, 2012, ID 928783, 17 pages.
2011
1. Kolpashchikov D.M., Gerasimova Y.V., Khan M.S. (2011) DNA nanotechnology for nucleic acid analysis: DX motif-based sensor ChemBioChem, 12, 2564-2567.
2. Nguyen C., Grimes J., Gerasimova Y.V., Kolpashchikov D.M. (2011) Molecular beacon-based tricomponent probe for SNP analysis in folded nucleic acids, Chemistry: A European J. 17, 13052-13058.
2010
1. Gerasimova Y.V., Peck S. Kolpashchikov D.M. (2010) Enzyme-Assisted Binary Probe for Sensitive Detection of RNA and DNA. Chem Commun. 46: 8761-8763.
2. Grimes J., Gerasimova Y.V. and Kolpashchikov D.M. (2010) Real-Time SNP Analysis in Secondary Structure-Folded Nucleic Acids, Angew Chem Int Ed Engl. 49: 8950-8953.
3. Kolpashchikov D.M. (2010) Binary Probes for Nucleic Acid Analysis, Chem. Rev. 110, 4709-4723.
4. Gerasimova Y.V., Hayson A., Ballantyne, J., Kolpashchikov D.M. (2010) A Single Molecular Beacon Probe is Sufficient for the Analysis of Multiple Nucleic Acid Sequences, ChemBioChem, 11, 1762-1768.
5. Lake A., Shang S., Kolpashchikov D.M. (2010) Molecular Logic Gates Connected via DNA Four Way Junctions, Angew. Chemie Int. Ed. Engl., 49, 4459-4462.
6. Gerasimova Y.V., Cornett E. Kolpashchikov D.M. (2010) RNA Cleaving Deoxyribozyme Sensor for Nucleic Acid Analysis: the Limit of Detection, ChemBioChem, 11, 811-817. This paper was featured on the cover of 6th issue of ChemBioChem journal.
The University of Central Florida invention called the OWL2, is a low-cost hybridization sensor that accurately detects single nucleotide variations (SNVs) under conditions that other probes cannot: at room temperature and in folded analytes. Detecting and analyzing SNVs in secondary folded DNA and RNA sequences is essential to identifying genetic disorders, infectious diseases, and drug-resistant pathogens.
Cells have DNA that store genetic information in the form of three nucleotides (codon) that code for amino acids, the building blocks of proteins. When a single nucleotide is mutated—for example, from G (guanine) to A (adenine)—it is referred to as a single nucleotide polymorphism (SNP) or an SNV. Such mutations are responsible for various medical conditions ranging from heart disease to diabetes and cancer. Early detection is critical for effective treatment.
Technical Details: The UCF OWL2 is a hybridization sensor that produces a fluorescence signal only when it complexes with fully complementary DNA or RNA analytes. The OWL2 comprises the following:
Under experimental conditions, the OWL2 was able to differentiate SNV in folded DNA analytes in the range of 5 to 38 C. The UMB is specific to the sequence of the sensor and does not depend on the sequence of the analyte. Thus, it allows the sensor to remain cost-efficient—the molecular beacon needs no modification when adapting the sensor to another analyte.
Partnering Opportunity: The research team is seeking partners for licensing, research collaboration, or both.
OWL2: a molecular beacon-based nanostructure for highly selective detection of single-nucleotide variations in folded nucleic acids, Nanoscale, 15, Feb 27, 2023, 5735-5742, https://doi.org/10.1039/D2NR05590B
