Pavalko, F. M., T. M. Roberts and L. S. Holliday. 1988. Relationship between plasma membrane motility and substrate attachment in the crawling movement of spermatozoa from Caenorhabditis elegans. Cell Motility and the Cytoskel. 11:16-23.

Holliday, L. S., M. R. Bubb and E. D. Korn. 1993. Rabbit skeletal muscle actin behaves differently than Acanthamoeba actin when added to soluble extracts of Acanthamoeba castellanii. Biochem. Biophys. Res. Com. 196:569-575.

Gluck, S. L., R. D. Nelson, B. S. Lee, L. S. Holliday and M. Iyori. 1994. Properties of kidney plasma membrane vacuolar H⁺ATPases: proton pumps responsible for bicarbonate transport, urinary acidification, and acid-base homeostasis. in Organellar Proton ATPases. (N. Nelson, ed), R. G. Landes, Austin TX.

Holliday, L. S., A. D. Dean, J. E. Greenwald and S. L. Gluck. 1995. C-type natriuretic peptide increases bone resorption in 1,25-dihydroxyvitamin D₃-stimulated mouse bone marrow cultures. J. Biol. Chem. 270 (32):18983-18989.

Holliday, L. S. and T. M. Roberts. 1995. Isolation and partial characterization of 30 nm-wide particles present in pseudopods of spermatozoa from Ascaris suum. Biochem. Biophys. Res. Com. 208:1073-1079.

Gluck, S. L., L. S. Holliday, M. Iyori, T. Kostrominova and B. S. Lee. 1995. Renal plasma membrane vacuolar H⁺ ATPases: Properties and function in acid-base homeostasis. In: Molecular Endocrinology (Schlondorf/Bonventure Ed), Marcel Dekker, Inc., New York, NY, pp. 369-414.

Gluck, S. L., D M. Underhill, M. Iyori, L. S. Holliday, T. Y. Kostrominova and B. S. Lee. 1995. Biochemical regulation of proton secretion by the vacuolar H⁺ATPase of renal epithelial cells. In: DeSanto, NG and Capsso, G., Eds. Acid-Base and Electrolyte Balance: Molecular, Cellular and Clinical Aspects. Instituto Italiano per Glistudy Filosofici, Naples, Italy. pp. 29-42.

Gluck, S. L., D. M. Underhill, M. Iyori, L. S. Holliday, T. Y. Kostrominova and B. S. Lee. 1996. Physiology and biochemistry of the kidney vacuolar proton ATPases. Ann. Rev. Physiol. Volume 58:427-446.

Lee, B. S., L. S. Holliday, B. Ojikuta, I. Krits and S. L. Gluck. 1996. Osteoclasts from various sources overexpress the B2 isoform of the vacuolar H+ATPase. Am. J. Physiol. 270 (Cell Physiol. 39): C382-C388.

Gluck, S. L., M. Iyori, L. S. Holliday, T. Kostrominova and B. S. Lee. 1996. Distal urinary acidification from Homer Smith to the present. Kidney Inter. 49:1660-1664.

Holliday, L. S., H. G. Welgus, C. J. Fliszar, G. M. Veith, J. J. Jeffrey and S. L. Gluck. 1997. Initiation of osteoclast bone resorption by interstitial collagenase. J. Biol. Chem. 272(35):22053-22058.

Corbett, J. W., J. S. Kerr, P. Wong, P. Harlow, G. A. Boswell, L. Pease, B. S. Lee, L. S. Holliday and S.L. Gluck. 1997. 2-sulfinylbenzothizoles,-oxazoles and -imidizoles are novel in vitro bone anti-reabsorptive compounds. Med. Chem. Res. 7(3):151-167.

Holliday, L. S., A. D. Dean, R. H. Lin, J. E. Greenwald and S. L. Gluck. 1997. Low NO concentrations inhibit osteoclast formation in mouse marrow cultures by cGMP-dependent mechanism. Am. J. Physiol. 272 (Renal Physiol. 41): F283-F291.

Gluck. S.L., B.S. Lee, S-P. Wang, D. Underhill, J. Nemota and L. S. Holliday. 1998. Plasma membrane V-ATPases in proton-transporting cells of the mammalian kidney and osteoclast. Acta Physiol. Scand. 163, supp 643, 203-212.

Lee, B. S., L. S. Holliday, I. Krits and S. L. Gluck. 1999. Vacuolar H+-ATPase activity and expression in mouse bone marrow cultures. J. Bone Miner Res. 12:2127-2136.

Lee, B. S, S. L. Gluck and L. S. Holliday. 1999. Interaction between the vacuolar H⁺-ATPase and microfilaments during osteoclastic bone resorption. J. Biol. Chem. 274:29164-29171.

Witten, P. E., L. S. Holliday, G. Delling and B. K. Hall. 1999. Immunohistochemical identification of a vacuolar proton pump (V-ATPase) in bone resorbing cells of an advanced teleost species (Oreochromis niloticus, Teleostei, Cichlidae). J. Fish Biology. 55:1258-1272.

Holliday, L. S., M. Lu, B. S. Lee, R. D. Nelson, S. Solivan, L. Zhang and S. L. Gluck. 2000. The amino-terminal domain of the B subunit of the vacuolar H⁺-ATPase contains a filamentous actin binding site. J. Biol. Chem . 275:32331-32337.

Holliday, L.S., S. L. Gluck, E. Slatopolsky and A. J. Brown. 2000. 19-Nor-1, 25- dihydroxyvitamin D₂ is less active than 1, 25-dihydroxyvitamin D₃ in stimulating bone resorption in mouse marrow cultures. J. Am. Soc. Nephrol. 11:1857-1864.

Lu M, L.S. Holliday, L. Zhang, W.A. Dunn Jr, and S. L. Gluck. 2001. Interaction between aldolase and vacuolar H⁺-ATPase: evidence for direct coupling of glycolysis to the ATP-hydrolyzing proton pump. J. Biol. Chem. 276:30407-30413.

Lu, M., S. Vergara, L. Zhang, L. S. Holliday, J. Aris and S. L. Gluck. 2002. The amino-terminal domain of the E subunit of V-ATPase interacts with the H subunit and is required for V-ATPase function. J. Biol. Chem 277:38409-38415.

Krits, I., R. B. Wysolmerski, L. S. Holliday, and B. S. Lee. 2002. Distribution of myosin II isoforms in resting and activated osteoclasts. Calcified Tissue Inter. 71:530-538.

Lee, B.S., S. C. Lee and L. S. Holliday. 2003. Biochemistry of mechanoenzymes: Biological motors for nanotechnology. Biomedical Microdevices: BioMEMS and Biomedical Nanotechnology, 5:269-280.

Jiang, J. J. Zuo, I. R. Hurst and L. S. Holliday. 2003. The synergetic effect of petidoglycan and lipopolysaccaride on osteoclast formation. Oral Surg Oral Med Oral Path Oral Rad Endod. 96: 738-743.

Holliday, L. S., A. Vakani, L. Archer and C. Dolce. 2003. Effects of matrix metalloproteinase inhibitors on bone resorption and orthodontic tooth movement. J. Dental Res. 82:687-691.

Dolce, C., A. Vakani, L. Archer, J.A. Morris-Wiman and L. S. Holliday. 2003. Effects of echistatin and an RGD peptide on orthodontic tooth movement in rats. J. Dental Res. 82:682-686.

27. Holliday, L. S., H. G. Welgus, J. Hanna, B. S. Lee, M. Lu, J. J. Jeffrey and S. L. Gluck. 2003. Interstitial collagenase activity triggers osteoclasts to form actin rings and ruffled membranes. Calcified Tissue. Inter. 72:206-214.

Jiang, J., J. Zuo, S-H. Chen and L. S. Holliday. 2003. Calcium hydroxide reduces LPS stimulated osteoclast formation. Oral Surg Oral Med Oral Path Oral Rad Endod. 95:348-354.

29.  Brown, A.J., C. S. Ritter, L. S. Holliday, J.C. Knutson, and S.A. Strugnell. 2004. Tissue distribution and activity studies of 1,24-dihydroxyvitamin D(2), a metabolite of vitamin D(2) with low calcemic activity in vivo.  Biochemical Pharmacol.  68:1289-1296.

Beem, E., L. S. Holliday, and M. S. Segal. 2004. The 1.4 MD apoptosome is a critical intermediate in apoptosome maturation. Am. J. Physiol. (Cell Phys.) 287:C664-C672.

Zuo, J. J. Jiang, C. Dolce and L. S. Holliday. 2004. Effects of basic fibroblast growth factor on osteoclasts and osteoclast-like cells. Biochem. Biophys. Res. Com. 318:162-167.

Hurst, I. R., J. Zuo, J. Jiang and L. S. Holliday. 2004. Actin-related protein 2/3 complex are required for actin ring formation in osteoclasts. J. Bone Miner. Res. 19:499-506.

Lu, M., Y.Y. Sautin, L. S. Holliday, and S. L. Gluck. 2004. The glycolytic enzyme aldolase mediates assembly, expression and activity of vacuolar H⁺-ATPase. J. Biol. Chem. 279:8732-8739.

Chen, S-H., M. R. Bubb, E. G. Yarmola, J. ,Zuo, J., Jiang, B. S. Lee, M. Lu, S.L. Gluck, I. R. Hurst, and L. S Holliday. 2004. Vacuolar H⁺-ATPase binding to microfilaments: Regulation in response to phosphatidylinositol 3-kinase activity and detailed characterization of the actin binding site in subunit B. J. Biol. Chem. 279:7988-7998

Holliday, L. S., M. R. Bubb, J. Jiang, I. Rita Hurst, J. Zuo. 2005. Interactions between V-ATPases and microfilaments in osteoclasts. J. Bioenergetics and Biomembranes. 37:419-423.

Dolce, C. and L. S. Holliday. 2006. Toward molecular orthodontics. In Essentials for Orthodontic Practice (Riola and Avery, ed), EFOP Press, Inc., Ann Arbor and Grand Haven, MI.

37. McMichael B. K., P. Kotadiya, T. Singh , L.S. Holliday, and B.S. Lee. 2006. Expression and subcellular distribution of tropomyosin isoforms in osteoclasts Bone 39:694-705.

Zuo, J., J. Jiang, S-H. Chen, S. V. Vergara, Y. Gong, J. Xie, H. Huang, M. Kaku and L. S. Holliday. 2006. The actin binding activity of subunit B of vacuolar H+-ATPase is involved in its targeting to ruffled membranes of osteoclasts. J. Bone Miner. Res. 21: 714-721.

39. Zuo, J., Archer,L.A., Cooper, A., Johnson,K., Holliday, L. S. and C. Dolce. 2007. Nuclear Factor κ B p65 Phosphorylation in Orthodontic Tooth Movement. J. Dent. Res. 86:556-9.

Zuo J, Vergara S, Kohno S, Holliday LS. 2008 Biochemical and functional characterization of the actin-binding activity of the B subunit of yeast vacuolar H+-ATPase. J Exp Biol. Apr;211(Pt 7):1102-8.

Pillagi, R., K. Antony, J. Zuo and L. S. Holliday. 2009. Propolis inhibits osteoclastogenesis. Dental Traumatology. 25:584-588.

Downey ME, Holliday LS, Aguirre JI, Wronski TJ. 2009 In vitro and in vivo evidence for stimulation of bone resorption by an EP4 receptor agonist and basic fibroblast growth factor: Implications for their efficacy as bone anabolic agents. Bone. Feb;44(2):266-74.

Holliday LS, Ostrov DA, Wronski TJ, Dolce C. 2009. Osteoclast polarization and orthodontic tooth movement. Orthod Craniofac Res. May;12(2):105-12. Review.

Ostrov DA, Magis AT, Wronski TJ, Chan EK, Toro EJ, Donatelli RE, Sajek K, Haroun IN, Nagib MI, Piedrahita A, Harris A, Holliday LS. 2009. Identification of enoxacin as an inhibitor of osteoclast formation and bone resorption by structure-based virtual screening. J Med Chem. 52:5144-5151.

Serrano EM, Ricofort RD, Zuo J, Ochotny N, Manolson MF, Holliday LS. 2009. Regulation of vacuolar H(+)-ATPase in microglia by RANKL. Biochem Biophys Res Commun. 389:193-197.

Pauley, K.M., M. Satoh, P.R. Dominguez-Gutirrez, S.M. Pop, L. S. Holliday, W.H. Reeves, and E.K.L. Chan. 2010. GW/P body assembly is induced in human monocytes in response to lipopolysaccharide stimulation of the innate immune signaling pathway. Immunology and Cell Biology 88:205-212.

Dolce, C. L. S. Holliday and J. K. Neubert. 2011 Prospects for medicinal orthodontics. In Essentials for Orthodontic Practice (Riola and Avery, ed), EFOP Press, Inc., Ann Arbor and Grand Haven, MI.

Neubert, J. K., R. M. Caudle, C. Dolce, E. J. Toro, Y. Bokrand-Donatelli, and L. S. Holliday. 2011. Neural modulation of orthodontic tooth movement. In Orthodontics (Mandic, ed), INTECH, Open Access Publisher, Rijeka, Croatia.

Xu, J., S. Barone, H. Li, L. S. Holliday, K. Zahedi and M. Soleimani. 2011. Slc26a11, a chloride transporter, localizes with the vacuolar H(+)-ATPase of A-intercalated cells of the kidney. Kidney Int. 80(9):926-937. (This manuscript was featured in a commentary that accompanied the article. Eladari D. and J. Teulon. 2011. A new regulator of the vacuolar H(+)-ATPase in the kidney. Kidney Int. 2011 Nov;80(9):907-9.)

Holliday, L.S. 2012. Vacuolar H⁺-ATPase: Targeting a “Housekeeping” Enzyme for Drug Development. Curr. Prot. Peptide Sci. 13: 105-106.

Toro, E. J., D. A. Ostrov, T. J. Wronski, and L. S. Holliday. 2012. Rational identification of a novel V-ATPase-directed inhibitor of bone resorption. Curr. Prot. Peptide Sci. 13: 180-191. Invited Review.

Toro, E. J., J. Zuo, D. Catalfamo, V. Bradaschia-Correa, V. Arana-Chavez, R. Donatelli, J.K. Neubert, T.J. Wronski, D. A. Ostrov, S.M. Wallet² and L. S. Holliday. 2012. Enoxacin directly inhibits osteoclastogenesis without inducing apoptosis. J. Biol Chem. 287:17894-17904.

Kim SS, DW Kwon, I Im, YD Kim, DS Hwang, LS Holliday, RE Donatelli, WS Son, ES Jun. 2012. Differentiation and characteristics of undifferentiated mesenchymal stem cells originating from adult premolar periodontal ligaments. Korean J Orthod.42(6):307-317.

Holliday, L.S., Huynh, N., Zuo, J., E.J. Toro. 2013. Mechanisms controlling vacuolar H+-adenosine triphosphatase activity:targets for the development of new therapeutic agents for the management of osteoporosis. Research and Reports in Biochem. 3:1-17. Invited Review.

Toro, E.J., Zuo, J., Guiterrez, A., La Rosa R, Gawron, A. , Bradaschia-Correa, V., Arana-Chavez, V., Dolce, C., Rivera, M. F., Lakshmyya, K., Bhattacharyya, I., Neubert, J.K., and Holliday, L.S. 2013. Bis-enoxacin inhibits bone resorption and orthodontic tooth movement. J. Dent Res. 92(10):925-931.

Rodriguez, D., E., Thula,T., Toro E. J., Yeh, Y-W., Holt, C., Holliday, L. S., and Gower L. B. 2014. Multifunctional role of osteopontin in directing intrafibular mineralization of collagen and activation of osteoclasts. ACTA Biomaterialia. 10(1):494-507.

Holliday, L.S. 2014. V-ATPase: An essential multitasking enzyme in physiology and pathophysiology. New Journal of Science. 2014. Article ID 675430, 21 pages.

Neiva, K, Catalfamo, D., Holliday, L. S., Wallet, S. M., and R.Pileggi. 2014. Propolis decreases LPS-induced inflammatory mediators in pulp cells and osteoclasts. Dental Traum. Epub Feb 6.

Rivera, M.F., Lee, J.-Y., Chukkapalli, S., Velsko I., Bhattacharyya, I. ,. Toro, E.J., Holliday, L. S. , and K. Lakshmyya. 2014. Bis-enoxacin blocks rat alveolar bone loss in polymicrobial periodontitis. PLOS One, 9(3):e92119. PMID:24638087.

Rody, W.J., Holliday, L. S., McHugh, K.P., Wallet, S.M., Spicer, V., Krokhin, O., 2014. Mass spectrometry anlaysis of gingival crevicular fluid in the presence of external root resorption. AJO-DO. 145(6):787-798. PMID: 24880850.

Oktay, S, Chukkapalli, S. S., Rivera, M. F., Velsko, I. M., Holliday,L. S., Kesavalu,L. 2014. Periodontitis in Rats Induces Systemic Oxidative Stress which is Controlled by Bone-targeted Antiresorptives. J. Periodontol. In press July 28, 2014.

Zuo, J., Toro, E.J., McHugh, K.P. Neubert J.K. and L. S. Holliday. 2015. FAM21 is required for osteoclastogenesis and is associated with specialized sorting of V-ATPase. In prep..

Huynh, N., VonMoss, L., Toro, E.J., McHugh, K.P.and L. S. Holliday. 2015. Osteoclast-derived exosomes are novel regulators of osteoclastogenesis. In prep.

Dolce C. and L.S. Holliday. 2015. Computational chemistry: A new frontier in orthodontics. In The 41^st Moyers Symposium (eds Kris De Koster)

Search Google Scholar for Lexie Holliday OpenAlex Works Search

Peer Review Positions

Young Investigator Awards, Canadian Arthritis Foundation; 2012 - 2012

AMS, NSF, NIH-NIAMS; 2008 - 2010

Journal of Bone and Mineral Rsearch, ASBMR; 2006 - 2011

ZAR1, NIAMS; 2005 - 2005

Professional Presentations

V-ATPase interactions with the cytoskeleton; FASEB Summer Research Conference-Transport ATPases:Genomics, mechanisms and relevance to disease. ; FASEB; 2005

Regulators of cytoskeletal and membrane dynamics in osteoclasts.; Dept of Orthopaedics seminar; Dept of Orthopaedics, Washington University of St. Louis; 2006

Osteoclast polarization and orthodontic tooth movement; COAST meeting; The Consortium on Orthodontic Advannces in Science and Technology; 2008

Rational design of a novel anti-osteoporotic bisphosphonate; Phil Griffin Distinguished Lecture Series; UFAA; 2010

Novel small molecule inhibitors of bone resorption; IADR symposium, Barcelona Spain; International Association of Dental Resarch; 2010

Novel small molecule inhibitors of bone resorption; Invited Seminar, Thousand Oaks CA; Amgen; 2010

Specialized V-ATPase sorting mediated by the WASH complex as a potential therapeutic target.; FOR-1406 Workshop V-ATPase as a therepeutic target; Ludwig-Maxamillions Universitat-Munchen Germany; 2012

Regulation of bone remodeling by exosomes; ASEMV meeting Asilomar; American Society for exosomes and microvesicles; 2014

Presentations

Role of exosomes in regulating bone remodeling and their potential for diagnosing and treating bone disease.

I will provide a general introduction to exosomes/extracellular vesicles and their emerging role in intercellular communicaiton. Their role in helathy tissues and pathological states including cancer will be described. Then I will discuss our own findings in how exosomes derived from osteoclasts appear to be involved in maintaining healthy bone. Finally, I will discuss strategies for using exosomes for the treatment of bone disease.

Subject Areas:

Biotechnology Medical Sciences

Keywords:

Biotechnology Bone Disease Cancer Exosome Osteoporosis

Audience:

Adults

Duration:

1 hour or less

Fee:

Expenses Only

Bis-enoxacin: Development of a new drug for osteoporosis and bone cancer

Development of new therapeutic agents has until recently relied on luck, or brute force (testing hundreds of thousands of molecules to find one that works). Now supercomputer-based computational chemistry has reached sufficent levels of sophistication to allow rational drug design and development. In this presentation, I will decribe studies in my laboratory, which led to the identification of a new and better anti-resorptive for the treatment of osteoporosis and bone cancer. This is an example of what can be done in the area of rational drug design.

Subject Areas:

Biotechnology Medical Sciences Science

Keywords:

Bone Disease Cancer Cell Biology Computers Molecular Biology

Audience:

Adults

Duration:

1 hour or less

Fee:

Expenses Only

You might want to be a scientist

I never really considered a career in science while I was in high school, mostly because I didn't know anything about it. In this presentation I will talk about my life in science, from graduate school chasing nematode worms around in pig intestines...not for the squeemish...to my life now as a tenure member of the University of Florida faculty. Life in science is a lot different than what most students expect, and some may find in an attractive alternative to consider.

Subject Areas:

Biotechnology Medical Sciences Science

Keywords:

Benefits Career Downsides Lifestyle Requirements

Audience:

High School

Duration:

1 hour or less

Fee:

Expenses Only

You might want to be a scientist

I never really considered a career in science while I was in high school, mostly because I didn't know anything about it. In this presentation I will talk about my life in science, from graduate school chasing nematode worms around in pig intestines...not for the squeamish...to my life now as a tenured member of the University of Florida faculty. Life in science is a lot different than what most students expect, and some may find in an attractive alternative to consider.

Subject Areas:

Biotechnology Medical Sciences Science

Keywords:

Benefits Career Downsides Lifestyle Requirements

Audience:

High School

Duration:

1 hour or less

Fee:

Expenses Only