Abstract
The University of Central Florida invention relates to methods for detecting a target pathogen or antibody in biological samples. The disclosed methods include three embodiments and do not require labeling or conjugating of the detection reagent with the signaling molecule or substance to generate detectable signals. The first embodiment relates to using an unlabeled antibody reagent that is specific to the target pathogen. The second embodiment relates to using the pathogen as the detection reagent and the antibody as the target analyte. The third embodiment relates to using an unmodified signaling substance to detect the presence of inclusion bodies. The technology has been used to screen for cytomegalovirus infection in urine samples.