Ion mobility spectrometry-mass spectrometry (IMS-MS) is ideally suited to study co-existing, transient conformations of proteins and their complexes related to diseases because of its high sensitivity and speed of MS analysis.
Many existing results suggest that IMS-MS could accurately elucidate structures for these protein conformations in a high-throughput manner.
The present technology identifies how protein tertiary structures can be determined from IMS-MS data in an automated manner.
Advantages:
- IMS-MS requires a fraction of sample amounts and time
- Does not suffer from charge-state dependent protein dynamics in the gas phase
- Computationally efficient
- Automatized
Click here to watch an interview with Dr. Bleiholder: https://www.youtube.com/watch?v=G7etpbzsWtg
