Abstract
FIU inventors have developed novel reagents for the study of DNA topology
and topoisomerases. These tools are also useful for screening Anti-Cancer Drugs
and Antibiotic Targeting DNA topoisomerases. FIU inventors have created a
method to mass-produce a type of fluorescently labeled circular DNA molecules
with high yields to study DNA topology and topoisomerases by fluorescence
resonance energy transfer (FRET), and to screen anti-cancer drugs and
antibiotics targeting DNA topoisomerases.In a typical assay to study DNA supercoiling and
topoisomerases, agarose gel electrophoresis is usually used to resolve DNA
topoisomers produced in the reaction mixtures. Since gel electrophoresis is
time-consuming it is desirable to develop other assays, such as
fluorescence-based assays, to study DNA topology and DNA topoisomerases. The
novel fluorescently labeled circular DNA molecules provide a quick and low-cost
method to study DNA topology and topoisomerases through FRET.
Benefit
Provides tools to study DNA topology and topoisomerases in real time by FRETFacilitates the development of kits and reagents for high throughput screening (HTS) against DNA topoisomerases for anticancer drug and antibiotics discovery using FRETAllows for the production of fluorescence-labeled DNA molecules at milligram rangeProvides high quality of relaxed and supercoiled DNA molecules
Market Application
The immediate and future applications of this technology are to provide a type of novel reagents to study DNA topology and topoisomerases by FRET and a new method for high throughput drug screening against DNA topoisomerases for anticancer drug discovery and antibiotics discovery
Brochure
