Technology Profile

Novel Tools for DNA Topology and Topoisomerases Research

Description

Abstract

FIU inventors have developed novel reagents for the study of DNA topology and topoisomerases. These tools are also useful for screening Anti-Cancer Drugs and Antibiotic Targeting DNA topoisomerases. FIU inventors have created a method to mass-produce a type of fluorescently labeled circular DNA molecules with high yields to study DNA topology and topoisomerases by fluorescence resonance energy transfer (FRET), and to screen anti-cancer drugs and antibiotics targeting DNA topoisomerases.In a typical assay to study DNA supercoiling and topoisomerases, agarose gel electrophoresis is usually used to resolve DNA topoisomers produced in the reaction mixtures. Since gel electrophoresis is time-consuming it is desirable to develop other assays, such as fluorescence-based assays, to study DNA topology and DNA topoisomerases. The novel fluorescently labeled circular DNA molecules provide a quick and low-cost method to study DNA topology and topoisomerases through FRET.

Benefit

Provides tools to study DNA topology and topoisomerases in real time by FRETFacilitates the development of kits and reagents for high throughput screening (HTS) against DNA topoisomerases for anticancer drug and antibiotics discovery using FRETAllows for the production of fluorescence-labeled DNA molecules at milligram rangeProvides high quality of relaxed and supercoiled DNA molecules

Market Application

The immediate and future applications of this technology are to provide a type of novel reagents to study DNA topology and topoisomerases by FRET and a new method for high throughput drug screening against DNA topoisomerases for anticancer drug discovery and antibiotics discovery

Brochure

Research Terms

Biochemistry Genetics Biomedicine Chemistry

Associated Patents

US 9,890,416; US 10,150,987

Patent Number US 9,890,416; US 10,150,987
Patent Status Issued
Issue Date -

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