Abstract
The University of Central Florida invention is an imaging method and system that reshapes Total Internal Reflection Fluorescence (TIRF) excitation light into a ring-shaped beam, which is conjugated to the back focal plane of an objective. The output end of the device comprises multiple multi-mode fibers arranged around a spacer to form a single ring. The device enables TIRF illumination with incoherent or coherent sources, provided that the coherence of the source is degraded before inputting the annular bundle. The device requires no moving parts, provides high excitation power throughput, and can generate uniform, artifact-free TIRF illumination over a large field of view.
Benefit
Useful in quantitative fluorescence microscopy for imaging surface features with a high signal-to-background ratioCompatible with coherent or incoherent light sourcesHigh excitation power throughput with no complex components, moving partsProduces no shadowing artifacts during subcellular imaging and provides a large field of viewMarket Application
Live-cell imaging on biomarkers near the surfaceSingle-molecule fluorescence imagingHigh-throughput, large field-of-view cellular fluorescence imagingSuper-resolution fluorescence imagingPublications
Single-shot,
shadowless total internal reflection fluorescence microscopy via annular fiber
bundle, Optics Letters, Vol. 45, No. 23, 6470-6473, 1 December 2020.
Brochure
