Abstract
The University of Central Florida invention is a 3D-printed, multilevel, fully packaged, microfluidic platform with an innovative metallization approach using liquid metal. Cost-effective, scalable and customizable, the innovation offers enhanced multi-modality for multi-phase control in the microchamber microenvironment. The UCF technology provides a monolithically 3D-printed array of microchannels in a multilayer circuit with an integrated microchamber. Microchannels at different levels are conduits towards a centrally located 2.5D microelectrode array (MEA) to stimulate/record electrogenic spheroids electrically. The microchannels also serve as inlets/outlets for injecting/suctioning liquids (for example, samples, reagents). The microchamber allows for controlling/isolating the cultured microenvironment and perfusing gases (such as O2, CO2) for electroactive responses. The device was characterized with synthetic organoids under phosphate buffer saline (PBS) and sample gas (oxygen) injection. Impedance responses to the changing microenvironment were studied, as was multi-metallic sheet resistance. Barrier integrity and coverage of the synthetic organoid matrix are also determined from frequency analysis.
Partnering Opportunity
The research team is seeking partners for licensing, research collaboration, or both.
Stage of Development
Prototype available.
Benefit
Inexpensive, scalable and customizableOffers an easy way to study biological cells, tissues and electrophysiological responsesNew way to integrate electrical, biological and phase control for chemical assaysMarket Application
Controlled microchambers for biological analysisHypoxia studies of spheroids, organoids and assembloidsDrug delivery and screening systemsPublications
- NanoFlorida 2023 Conference. Oral Presentation. Orlando, FL, USA. March 3-5, 2023
- Transducers 2023 International Conference on Solid-State Sensors, Actuators and Microsystems. Oral Presentation and Paper. Kyoto, Japan. June 25-29, 2023
Brochure
