Enables Single Vesicle DNA/RNA Sequencing to Differentiate Sub-Populations of Extracellular Vesicles (EVs)
These 3D-printed extracellular vesicle (EV) droplets enable DNA and RNA sequencing to differentiate extracellular vesicle subpopulations. Extracellular vesicles are nanosized vesicles containing a variety of cargo, including mRNA, proteins, and lipids, that nearly all cell types release. EVs are essential to many physiological processes, such as coagulation, inflammatory response, cell maturation, adaptive immune response, bone calcification, and neural cell communication. They also play fundamental roles during cancer progression, suppressing immune surveillance in the tumor microenvironment and establishing the premetastatic niche. Due to the EVs’ ability to transfer their contents to cells in the body and their stability in the bloodstream, there is broad translational potential for EVs in oncology, such as tumor biomarkers. However, the current single-vesicle analysis uses bulk approaches, creating heterogenous EV samples in vesicle structure, contents, and function. This heterogeneity impedes EV development as a biomarker.
Another issue is current microfluid technologies. These technologies form droplets once a single cell enters a fluidic orifice, creating “drops on demand” containing single cells. Current microfluidic technologies at the nanoscale are carefully controlled and are susceptible to low throughput and clogging. Current systems cannot detect EVs beyond a certain threshold; thus, creating single-cell EV droplets is not feasible.
Researchers at the University of Florida have developed 3D-printed aqueous droplets containing extracellular vesicles (EVs) for sequencing the DNA and RNA of single-cell EVs. DNA/RNA sequencing of an individual EV can identify its nucleic acid contents, including structure, composition, and contents for performing a specific function. By characterizing an EV at a single-vesicle resolution level, EV subpopulations of composition and content are differentiated from a heterogenous sample, closing the gap to EVs’ potential as a biomarker. The global EV market was estimated to be worth $169M in 2023 and is poised to reach $356 M by 2028, growing at a CAGR of 16% from 2023-20281.
Application
DNA and RNA sequencing of individual extracellular vesicles (EVs) via 3D printing the EVs within aqueous droplets, differentiating EV subpopulations
Advantages
- Enables DNA/RNA sequencing of individual vesicles, differentiating sub-populations from a heterogenous sample of EVs masked by bulk sequencing approaches
- Characterizes the entirety of the nucleic acid contents of EVs at single vesicle resolution, enabling the identification of biomarkers for evaluating health or early detection of diseases
- Prints and traps the droplets into a stable, solid-like medium, avoiding the barriers and pitfalls of channel-based micro- and nano-fluidic approaches, such as poor throughput, clogging, and agglomeration of the particles
Technology
This technology uses a 3D printing apparatus to form an aqueous droplet containing a single extracellular vesicle (EV) and a single DNA-barcoded microbead, enabling DNA/RNA sequencing within each EV. A drop of aqueous solution contains an EV and microbead for performing biochemical reactions. The droplet is 3D printed on a medium via a nozzle connected to a supply of the aqueous solution the droplets are made of. Once on the medium, the biochemical reaction occurs within the droplet, and the droplets are separated or pooled from the medium via centrifugation. The aqueous phase with the droplet contents is then removed from the medium. This technology addresses EV heterogeneity and enables the composition, contents, and function identification of extracellular vesicle subpopulations.
Brochure
